what causes a protein to migrate on SDS-PAGE in such a way that it appears to be a protein that is much bigger

Answers

Answer 1

Answer: SDS-PAGE is used for separation of proteins or protein fragments based on their size. SDS is an ionic detergent that denatures and binds to proteins. Consequently, the proteins are negatively charged. An electric current is running through the gel in the electrophoresis with positive pole on the bottom. Since proteins are negatively charged, they migrate toward the positive pole of the gel. Some of the proteins are smaller so they can travel more quickly through the gel than the bigger proteins.

Related Questions

An unnatural condition or an infection that causes your body to function poorly is a(n)emotion disease hangover stressor
A fossil is found that shows the organism that produced it had a skeleton and also suggests that the organism may have had hair covering it's body. Based on this evidence, it can also be assumed that the organism is a member of the domain
With the exception of some sponges, all animals are _____. coelomates autotrophs heterotrophs hermaphrodites
Translation of mRNA into segments of tRNA allows which organic molecules to combine to form proteins at the ribosome?
What is the study of the human body

Jordan is a 14 year old middle school student, weighing 275 pounds and is 5’6” tall. Over the past 2 years, he has gained 60 pounds, as he has begun to withdraw from social activities, and avoid other students, due to bullying from others his age about his weight/appearance. Lately, Jordan has been missing a great deal of school too, particularly on the days he has gym. Jordan’s parents are both average in height and weight, and Jordan’s mother says that he just takes after his grandfather William, who “was a husky man, and died of a sudden heart attack at the age of 44”. She says “We just have fat genes in the family; you can’t do anything about that”! He has been told he had “no willpower, or is weak, or that he needed to change his lifestyle, eat less and exercise more.” Jordan says “I go for a couple days without eating, but then I get so hungry that I could eat the couch”! You are the school nurse doing height and weight assessments on the middle school group this year. How would you assess and advise Jordan?

Answers

As a part of the height and weight assessment, the school nurse should analyse and evaluate Jordan's BMI, following which proper advice has to be given regarding his weight management.

Jordan's BMI can be calculated as:

BMI = Weight (in kg) ÷ Height² (in m)

From the given data, his weight and height are:

Weight = 275 pounds = 124.74 kg

Height = 5'6'' = 1.68 m

Therefore, his BMI will be = $(124.74)/((1.68)^2)$ = 44.2

According to the calculated BMI, Jordan falls under the category of obesity grade II. This puts him at a risk of lifestyle disorders such as cardiovascular diseases, diabetes, and hypertension.

Jordan must be made aware of the consequences of his weight as well as his eating habits. He should be advised on a proper diet to be followed for reduction of weight and prevention of diseases.

As he has started withdrawal and avoidance, he should also be encouraged to seek counselling. His parents and friends can also be involved in this process, which will provide him with the required motivation and not criticism.

Learn more about weight management in:

brainly.com/question/30197331

#SPJ2

Going without eating isn't a solution. For one, that just makes you more hungry and more likely to eat junk food. Secondly, if your body doesn't receive new nutrients, it will start to save the energy it already has. This makes you less likely to lose weight.

Instead of not eating altogether, eat healthy snacks that are high in protein and low in carbohydrates and fat. Vegetables, nuts, and fruits are all great snacks. Come to school more often on gym days, to help you get more exercise, and spend about an hour outside doing physical activities every day. Go for a walk around your neighborhood.

Ignore the bullies, they just feel bad about themselves and take it out on you.

What if FGF21? What is FAP? why was it chosen as a potential target for anti-diabetic therapy? what results suggest that this might be problematic, i.e., that the response might not be what was hoped?

Answers

FGF21 is Fibroblast Growth Factor 21 and FAP is Fibroblast Activation Protein. It has been said that it can lower blood glucose and raise insulin responsiveness, however, only 40% agreed to it and the rest got additional anti-diabetic agent to provide a better control of glucose levels.

Complete the sentence Yo___ de Asuncion a. venir b.vienes c.vengo d.venis

Answers

yo vengo de Asuncion :)

Spanish: Yo vengo de Asuncion
English: I come from Asuncion

Steroids, cholesterol, and wax are all examples of?

Answers

Steroids, cholesterol, and wax are all examples of lipids.

Steroids, cholesterol, and wax are all examples of lipids

What should be done whenever you are finish using microscope

Answers

Remove the slide,return the scope to low powered, and turn off the light.

Hope this helps

Remove the slide , return the scope to low powered and turn off the light

Will mark brainiest Which of the following would be considered a biotic factor in an ecosystem?

flower

fog

soil

water

Answers

I am pretty sure the answer is flower because biotic factors are living things in an ecosystem. Seeing as how fog, soil, and water are not living the answer should be flower.

Answer:

FLOWER

Explanation:

ME IS SMART

Other Questions

The _____ theory is an evolutionary concept that suggests that behaviors that help an organism survive are passed on while weak behaviors are lost.genetic driftgene flowoptimalityoptional

Protozoans that are parasites never have more than one host? true or false

Choices made by people and nations impact and shape American History.Which statement about Reconstruction supports this enduring understanding? A. Reconstruction legislation changed American attitudes regarding race and ended discrimination in society. B. Reconstruction policies brought Americans with opposing viewpoints together and resolved sectional differences in society. C. Reconstruction produced three Constitutional amendments that have influenced American society to the present day. D. The success of Reconstruction showed that the president and Congress can work together in reaching a compromise.

Which mechanism do sponges possess that makes a predator think twice before attacking it?