You want to prepare a solution with a concentration of 200.0μM from a stock solution with a concentration of 500.0mM. At your disposal, you have 5-mL and 10-mL transfer pipets and volumetric flasks of sizes 100, 250, 500, and 1000mL. Which of the following serial dilutions will give you the 200.0μM solution?

Answers

Answer 1
Answer:

Answer:

1) The dilution scheme will result in a 200μM solution.

2) The dilution scheme will not result in a 200μM solution.

3) The dilution scheme will not result in a 200μM solution.

4) The dilution scheme will result in a 200μM solution.

5) The dilution scheme will result in a 200μM solution.

Explanation:

Convert the given original molarity to molar as follows.

500mM = 500mM * ((1M)/(1000M))= 0.5M

Consider the following serial dilutions.

1)

Dilute 5.00 mL of the stock solution upto 500 mL . Then dilute 10.00 mL of the resulting solution upto 250.0 mL.

Molarity of 500 mL solution:

M_(2)= (M_(1)V_(1))/(V_(2))= ((0.5M)(5.00mL))/(500 mL)= 5 * 10^(-3)M

10 mL of this solution is diluted to 250 ml

M_(final)= (M_(2)V_(2))/(V_(final))= ((5 * 10^(-3)M)(10.0mL))/(250 mL)= 2 * 10^(-4)M

Convert μM :

2 * 10^(-4)M = (2 * 10^(-4)M)((1 \mu M)/(10^(-6)M))= 200 \mu M

Therefore, The dilution scheme will result in a 200μM solution.

2)

Dilute 5.00 mL of the stock solution upto 100 mL . Then dilute 10.00 mL of the resulting solution upto 1000 mL.

Molarity of 100 mL solution:

M_(2)= (M_(1)V_(1))/(V_(2))= ((0.5M)(5.00mL))/(100 mL)= 2.5 * 10^(-2)M

10 mL of this solution is diluted to 1000 ml

M_(final)= (M_(2)V_(2))/(V_(final))= ((2.5 * 10^(-2)M)(10.0mL))/(1000 mL)= 2.5 * 10^(-4)M

Convert μM :

2.5 * 10^(-4)M = (2.5 * 10^(-4)M)((1 \mu M)/(10^(-6)M))= 250 \mu M

Therefore, The dilution scheme will not result in a 200μM solution.

3)

Dilute 10.00 mL of the stock solution upto 100 mL . Then dilute 5 mL of the resulting solution upto 100 mL.

Molarity of 100 mL solution:

M_(2)= (M_(1)V_(1))/(V_(2))= ((0.5M)(10mL))/(100 mL)= 0.05M

5 mL of this solution is diluted to 1000 ml

M_(final)= (M_(2)V_(2))/(V_(final))= ((0.05M)(5mL))/(1000 mL)= 0.25 * 10^(-4)M

Convert μM :

0.25 * 10^(-4)M = (0.25 * 10^(-4)M)((1 \mu M)/(10^(-6)M))= 25 \mu M

Therefore, The dilution scheme will not result in a 200μM solution.

4)

Dilute 5 mL of the stock solution upto 250 mL . Then dilute 10 mL of the resulting solution upto 500 mL.

Molarity of 250 mL solution:

M_(2)= (M_(1)V_(1))/(V_(2))= ((0.5M)(5mL))/(250 mL)= 0.01M

10 mL of this solution is diluted to 500 ml

M_(final)= (M_(2)V_(2))/(V_(final))= ((0.01M)(10mL))/(500 mL)= 2 * 10^(-4)M

Convert μM :

2 * 10^(-4)M = (2 * 10^(-4)M)((1 \mu M)/(10^(-6)M))= 200 \mu M

Therefore, The dilution scheme will result in a 200μM solution.

5)

Dilute 10  mL of the stock solution upto 250 mL . Then dilute 10 mL of the resulting solution upto 1000 mL.

Molarity of 250 mL solution:

M_(2)= (M_(1)V_(1))/(V_(2))= ((0.5M)(10mL))/(250 mL)= 0.02M

10 mL of this solution is diluted to 1000 ml

M_(final)= (M_(2)V_(2))/(V_(final))= ((0.02M)(10mL))/(1000 mL)= 2 * 10^(-4)M

Convert μM :

2 * 10^(-4)M = (2 * 10^(-4)M)((1 \mu M)/(10^(-6)M))= 200 \mu M

Therefore, The dilution scheme will result in a 200μM solution.


Related Questions

Write your answer in scientific notation and Stanford form.​
What are some examples of exchange reactions ?
A tank has 30 gallons of gasoline how many liters of gasoline are there in the tank
What mineral is this? Irregularly shaped, white with nonmetallic luster, the hardness of 2.5, shows cubic cleavage and dissolves easily in water.
Why do the particles of objects have both kinetic and potential energy?  

as the bright sun shines upon the water the water slowly disappears the same sunlight gives energy to the surrounding plants to convert water and carbon dioxide into sugar and only oxygen gas which change is a physical and which is chemical

Answers

Well when the sun makes water dissaper thats usually evaporation.

The chemical changes is when the plants absorb the energy from the sunlight and convert water and carbon dioxide into sugar and oxygen gas. Physically nothing has changed. The plant still looks the sam changes occur internally these internal changes are chemical in nature. 

What physical property makes metal pots good for cooking?

Answers

Answer is: heat conductivity.

Thermal conductuction is the transfer of heat through physical contact. Thermal conduction is the transfer of heat by microscopic collisions of particles. Heat spontaneously flows from a hotter to a colder body.

The process of heat conduction depends on four basic factors: the temperature gradient, the cross section of the materials involved, their path length and the properties of those materials.

Metallic bond increace electrical and thermal conductivity.  

Metals conduct heat, because when free moving electrons gain energy (heat) they vibrate more quickly and can move around.  


Metal pots are good for cooking because they have heat conductivity.

Shield volcano type of eruption?


Answers

Shield volcanoes are the larges volcanoes in the world, named for their flat, shield-like structure. Their eruptions are highly fluid, so the lava travels quite fast. This results in steady accumulation of sheets of lava, building up the volcanoes form. Eruptions contain low viscosity magma. This means that the magma is relatively "thin", and it will flow quickly. 

Hope this helps! 

it is not a big explosive eruption ti is normally a small eruption
  fun fact
 moist of haiwy is a shield volacono

Name three types of bodies in the solar system

Answers

Sun, the plants, dwarf plants. you could also say gas giants.
Planets,stars, moons and a lot of more bodies in the solar system

What really is the Ebola virus?

Answers

The Ebola virus belongs to a family of viruses termed Filoviridae. Filovirus particles form long sometimes branched filaments of varying shapes, as well as shorter filaments , and may measure up to 14,000 nanometers in length with diameter of 80 nanometers.Viral particles contain one molecule of single stranded RNA enveloped in a lipid membrane. New viral particle bud from the surface of their host cell. Although Ebola virus was only discovered in 1976, it is an ancient virus and is thought to have split from other viruses thousands of years ago.

The Ebola virus is a highly infectious and often deadly viral disease that belongs to the Filoviridae family.

It was first identified in 1976 during two simultaneous outbreaks in Sudan and the Democratic Republic of Congo (formerly Zaire). The virus is named after the Ebola River, near the location of the Congolese outbreak.

Ebola is transmitted to humans through direct contact with the blood, bodily fluids, or tissues of infected animals, such as fruit bats, monkeys, or gorillas. It can then spread from person to person through close contact with the blood, secretions, organs, or other bodily fluids of infected individuals.

Ebola causes severe symptoms, including high fever, headache, muscle and joint pain, fatigue, diarrhea, vomiting, and internal and external bleeding. The disease has a high fatality rate, ranging from 25% to 90%, depending on the strain of the virus and the healthcare resources available.

To learn more about the Ebola virus

brainly.com/question/836713

#SPJ8

Which state of matter has the least amount of energy?

Answers

Solid has the least amount of energy. 

This is so because liquid is free to move and can fit in any shape. 
Gas floats and is usually always free to move.

Solid isn't free to move unless some force moves it.

Matter has the least energy as a solid