The scientist anneals a mutant oligonucleotide to the original M13 DNA. Then DNA polymerase replicates the M13 DNA. After DNA synthesis, the scientist adds a restriction enzyme, EcoRI, that recognizes the DNA sequence 5′−GAATTC−3′5′−GAATTC−3′ and cuts after the guanosine nucleotide. Following the restriction digest, the scientist adds exonuclease III for a short period of time. Another round of DNA polymerase‑catalyzed DNA synthesis completes the procedure. What is the nucleotide mixture required by the DNA polymerase reaction in this procedure?

Answers

Answer 1

Answer:

Answer:

The mixture of nucleotides required for this procedure is called dNTPs.

Explanation:

When the polymerase is used in a PCR procedure, to replicate a molecule or DNA fragment, use a combination of nucleotides called dNTPs.

DNTPs are nothing less than DNA nucleotides, applied as reagents in a PCR solution, which provide the material necessary for the polymerase to replicate DNA, even outside a biological system.

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A genetic engineer needs to use gene therapy to help a person with cystic fibrosis. Arrange the following steps in the order the engineer would use them.a. Use only the steps you need. Inject the modified CFRT gene into a fertilized egg and implant into a woman who will be the surrogate mother.b. Combine the cloned CFRT gene with a disarmed respiratory virus.c. Clone the CFRT gene from someone with cystic fibrosis.d. Clone the CFRT gene from someone without cystic fibrosis.e.Modify the CFRT gene by putting on a different promoterf. Test the patientâ€™s blood cell DNA with PCR to see if they have the CFRT transgene.g. Have the patient use an inhaler that contains the modified respiratory virus.
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Term Definition3. Biotic factor a. The nonliving factors in an environment4. Abiotic factor b. A group of ecosystems that share the same type of climate5. Biome c. All of the living organisms in an environment
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Although this moss plant undergoes alternation of generations and has two distinct body forms, its life cycle began as a A) seed. B) single cell. C) haploid gamete. D) cluster of cells.

Answers

The Answer fam is B) Single Cell

Hello to fully answer your question it would be (A. seed
If you need a more in depth explaination just comment down below

Signed by, Virtuoso Sargedog

Seeds of plants are the best example of which living characteristic

Answers

Answer:

adaptation through evolution and ability to reproduce

The secretion of hormones by the pituitary gland is an example of _______________.autocrine signaling
paracrine signaling
endocrine signaling
direct signaling across gap junctions

Answers

Answer:

The correct answer is the third option - endocrine signaling.

Explanation:

The endocrine signaling takes place when endocrine cell organs or glands release hormones that flow through the bloodstream and that act on distantly located target cells in the body. The endocrine system includes several glands that release or secretes different hormones.

The pituitary gland transfers signals to other endocrine glands to release or inhibit their hormone production according to the brain signals. The adrenocorticotropic hormone (ACTH) is released by the anterior pituitary gland that stimulates cortisol release in the adrenal glands in stressed conditions.

Thus, the correct answer is option third - endocrine signaling.

Final answer:

The pituitary gland secreting hormones is an example of endocrine signaling, where hormones are secreted directly into the bloodstream and carried to target cells.

Explanation:

The secretion of hormones by the pituitary gland is an example of endocrine signaling. Endocrine signaling refers to the process where glands secrete hormones directly into the bloodstream, which are then carried to the target cells elsewhere in the body. In contrast, autocrine signaling involves cells responding to their own signals, paracrine signaling refers to cells communicating with nearby cells, and direct signaling across gap junctions involves communication between adjacent cells through specific channels.

Learn more about Endocrine Signaling here:

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Atoms share electrons in which of the following?ionic bonds
hydrogen bonds
covalent bonds
van der Waals forces

Answers

Covalent bonds electrons are shared

PLEASE HELP NOW.... What are the four ingredients for roux A. Butter, gravy, milk, and turkey
B. Butter, chicken fat, flour, and turkey stock
C. Butter, turkey fat, flour, and turkey stock

Answers

C hdksjwkekeowkwhdjwiwk

Answer:

it is C i hope this helps

Which of the following organisms has a closed circulatory system? a. Sponges
b. Cnidarians
c. Nematodes
d. Insects
e. Vertebrates

Answers

Answer:

The correct answer is E. Vertebrates have a closed circulatory system.

Explanation:

The main mission of the circulatory system is to deliver oxygen and nutrients to all body cells.

In the closed circulatory system, blood travels inside a network of blood vessels, without leaving them. The material transported by the blood reaches the tissues through diffusion. It is characteristic of all vertebrates, including humans.

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Drosophila melanogaster have four pairs of chromosomes.a.what is the ploidy level at metaphase ii of meiosis?a. 8nb. 1nc. 4nd. 2ne. 0nb.what is the ploidy level at prophase of mitosis

I did the lab but I don’t know what goes in the table.

What is the significance of secreting inactive pepsinogen from the mucosal cells instead of pepsin?