The eggs of frogs are surronded by a

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Answer 1

Answer: In order to protect the eggs and developing embryos, frogs produce a thick, sticky coating surrounding the egg called the jelly coat. This jelly coat must be removed before the embryo can be effectively manipulated or microinjected. Jelly coats are important for X. tropicalis development. We have noticed that completely de-jellied embryos often have difficulty gastrulating properly. We believe this is a mechanical problem. The vitelline envelope of X. tropicalis embryos is loose and sticky. Once stuck to the bottom of the dish, it appears to interfere with normal gastrulation.However, simply coating the dishes with a thin layer of agarose prevents this problem. Therefore whenever we completely de-jelly the embryos, we raise them on agarose coated dishes until gastrulation is complete. Once gastrulation is completed, the embryos can be raised in regular petri dishes. If the embryos are not being microinjected, then a partial de-jelly can be very helpful. Often times due to the jelly coat, the embryos can be tightly stuck to each other. A partial de-jelly can separate the embryos and make it easy to transfer from dish to dish. The remaining jelly coat is enough to allow the embryos to gastrulate normally.To de-jelly X. tropicalis embryos, immerse in 3% cysteine made up in 1/9xMR (pH to ~7.5-8.0 with NaOH) or water. Swirl the embryos gently and the jelly coat will slowly be removed. NOTE: Embryos being dejellied before the first cleavage may develop secondary axes if the embryos are swirled too vigorously. Therefore, if the dejellying is being done at the one cell stage, very gentle or no swirling is recommended. For embryos past the first cleavage, we swirl the embryos every so often in an erlenmeyer flask which greatly speeds the process.For a partial de-jelly, immerse in cysteine just long enough until the embryos appear to be separate and sit next to each other in a monolayer. At this point the embryos should be easy to manipulate, and the jelly coat should still be visible around the embryo under the stereomicroscope. For a complete de-jelly, immerse in cysteine until the embryos pack tightly with each other without any clear space between the embryos. Again visualization under the stereomicroscope helps.

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A contraction that rhyme's with the opposite of narrow
Which sentence does not contain misplaced or dangling modifiers?A. The veterinarian, found in the woods with a broken leg, offered to help the dog. B. Found in the woods, the veterinarian offered to help the dog with a broken leg. C. Found in the woods with a broken leg, the veterinarian offered to help the dog. D. The veterinarian offered to help the dog found in the woods with a broken leg.
In the following sentence what is the main clause I generally prefer chocolate ice cream but vanilla ice cream with chocolate sauce is just as goodI generally prefer chocolate ice creamvanilla ice cream with chocolate sauce is just as goodboth of the above none of the above
Which sentence does not contain any errors in comma usage?A.The artist paints landscapes and animals and then she sells her artwork at the outdoor market.B.Many farmers planted corn, and others planted soy beans.C.Jeremy sent a letter in the mail, but, later that week Katie replied by e-mail.D.A factory recently closed in Pittsburgh but, another one is opening soon.
What does rural mean

When Angelou describes her memory of her first visit to Mrs. Flowers's home as "sweet-milk fresh," what is she suggesting about this remembered event? A. The important event changed her life for the better. B. The happy event involved sugary refreshments. C. The tiresome event required her to carry the groceries to the house. D. The unexpected event reminded her of her mother.

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When Angelou describes her memory of her first visit to Mrs. Flower's home as "sweet-milk fresh," what she is suggesting about this remembered event is that A. the important event changed her life for the better.

Which sentence is a run-on sentence? A. The cheetah is the fastest land animal; the sailfish is the fastest animal in water. B. A professional marathon runner can run 26 miles in just over two hours. C. The gazelle can only run about half as fast as the cheetah. D. Snails move very slowly, a banana slug can move 6 inches in two hours.

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The run on sentence is D. Snails move very slowly, a banana slug can move 6 inches in two hours.

A run-on sentence is a sentence that has at least two independent clauses which are not properly punctuated.

When two independent clauses are connected only by a comma, it is a run-on sentence that is called a comma-splice.

Run-on sentences can be corrected with the use of conjunctions (for, and, nor, but, or, yet, so)

Snails move very slowly, and a banana slug can move 6 inches in two hours. (corrected run-on sentence)

the answer is D. Snails move very slowly, a banana slug can move 6 inches in two hours

Where does Amory spend most of his time after his breakup with Rosalind?his job
his apartment
bars
the shows

Answers

Answer:

the bars

Explanation:

Answer:

The bar

Explanation:

He is depressed therefore drinking.

Which of the following methods of communication did Native American tribes use?smoke signals
cave drawings
handwritten letters
hieroglyphs

Answers

Answer:

The correct option is A) smoke signals

Explanation:

In the past, smoke signals were a common method for effective and visual communication over long distances. It uses puffs of smoke to generate a message.

The method of smoke signals simply implied that a fire is created and then covered by a blanket. When the cover was released, smoke would be created which could be seen over long distances.

Different fuels were used for making smokes and changes in the colour and density of smoke created different messages.

Answer:

A. Smoke signals is the answer

Explanation:

I took the lesson and I got it correct

Socratic Seminar HelpWhat topics do I use for
SOMEONE SHOULD NOT TAKE FULL RESPONSIBILITY FOR THEIR CREATION.

Answers

Answer:

Parents should not be held, nor take full responsibility of their children as adults.

Explanation:

When parents are raising their children from birth until adulthood, the parents should be held fully accountable for how their children are raised.

However, once your child/children reaches adulthood, THEY should be held fully responsible for themselves as individuals.

At this point, the parents cannot be held liable for their "adult" children's choices, actions, behaviors, etc. All the parents can do is put their faith in their children's lives, as they raised them, leaving the parents not fully responsible for their own creation, meaning their children.

We waited in a long line for the first showing of that new movie.Which one is the adjective and which one is the demonstrative adjective?

Answers

Answer:

Adj: long Demonstrative Adj: that

Explanation:

An adjective (adj) describes a noun. Line is a noun. Ask the question, "What type of line?" You then get the answer "long line."

Demonstrative Adj's: this, that, these, those.

"that" is the demonstrative adj

Hope this helps :)

Other Questions

Which word correctly completes the sentence? Do you see how those __________ tails swish back and forth as they walk?A. horses' B. horses C. horse's

Read the following short excerpt of student writing. Use your vocabulary and grammar knowledge to determine which suggestions you would make to improve his or her grammar and word choice.I really thought she loved me but I discovered her grave betrayal one afternoon. It was easy to figure out because she accidentally sent a text to me that was meant for him. I was confused by the text because it said, "Don't tell Mike." I felt insulted that she thought she could get away with this infidelity. At first I felt very angry bitter and hateful but then, I felt so hopeless and depressed. I longed for her to call me. I thought if I could just hear her pleasing voice, maybe then I would not be filled with such bitterness. Instead of calling her I talked to another friend who is good at listening and feeling my pain. Talking with her makes me feel euphoric. I think I may be on the road to recovery. Which comma rule does this student need to apply to this passage? I really thought she loved me but I discovered her grave betrayal one afternoon. It was easy to figure out because she accidentally sent a text to me that was meant for him. I was confused by the text because it said, "Don't tell Mike." I felt insulted that she thought she could get away with this infidelity. At first I felt very angry but then I felt so hopeless and depressed. Use a comma to separate three or more items in a series. Use a comma before a conjunction to separate two complete thoughts. Use a comma to set off a phrase or clause at the beginning of a sentence. Use a comma to separate coordinate adjectives.

I need expert quotes on red pandas

3. Is. your favorite painter?