For the Bradford assay, the instructor will make a Bradford reagent dye by mixing 50 ml of 95% v/v ethanol with 100 mg of Coomassie Blue followed by the addition of 50 ml of 85% v/v phosphoric acid. This entire mixture is then diluted to 1 liter with water. What is the final concentration of phosphoric acid?
Answers
Answer:
4,25% v/v H3PO4
Explanation:
The concentration of phosphoric acid (H3PO4) is expressed as a volume / volume percentage, which means:
%v/v H3PO4 = (mL of pure H3PO4/mL of solution)*100%
In other words, we are only interested in the final volume of the solution to which the phosphoric acid was diluted, regardless of its composition. Which in this case is 1 L (1000 mL).
We can then apply the following equation, commonly used to calculate the initial or final concentration (or volume) of a substance when it is diluted:
Ci*Vi=Cf*Vf
Where:
Ci, is the initial concentration of the substance.
Vi, the initial volume of the substance
Cf, the final concentration reached after dilution
Vf, the final volume of the solution at which the substance was diluted
In this case, the incognite would be the final concentration of H3PO4 reached after dilution, that is, Cf. Therefore, we proceed to clear Cf from the previous equation and replace our data:
Cf = (Ci*Vi)/Vf = (85% v/v * 50 mL)/1000 mL = 4,25 % v/v
Note that being up and down in the division, the mL unit is canceled to result in% v / v.
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A way in which chemistry affects how To use a computer is The colors in the computer display are the result of substances that glow. Option A
In modern computers, the display technology commonly used is Liquid Crystal Display (LCD) or Light Emitting Diode (LED) technology. These displays rely on the principles of chemistry to produce the colors that we see on the screen.
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LED screens, on the other hand, use arrays of light-emitting diodes to generate light. These diodes are made of semiconducting materials, often composed of compounds like gallium, arsenic, and phosphorus. When an electric current passes through these compounds, they emit light of a specific wavelength, resulting in the different colors displayed on the screen.
So, the colors we see on a computer display are a direct result of the chemical properties and behavior of the substances used in the LCD or LED technology. Chemistry plays a crucial role in the design and functionality of computer displays, enabling us to view vibrant and accurate colors while using our computers.
Option A
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Answers
Answer:
123.41 g
Explanation:
Given that the ethyl alcohol produced is 11.0 % by volume.
It means that 1000 mL contains 110 mL of ethyl alcohol
Given that the volume is:- 725 mL
So,
Volume of ethyl alcohol = = 79.75 mL
Given that:- Density = 0.789 g/cm³ = 0.789 g/mL
So, Mass = Density*Volume = = 62.92 g
Calculation of the moles of ethyl alcohol as:-
Molar mass of ethyl alcohol = 46.07 g/mol
The formula for the calculation of moles is shown below:
Thus,
According to the reaction:-
2 moles of ethyl alcohol is produced when 1 mole of glucose reacts
Also,
1.37 moles of ethyl alcohol is produced when mole of glucose reacts
Moles of glucose = 0.685 Moles
Molar mass of glucose = 180.156 g/mol
Mass = Moles*Molar mass = = 123.41 g
Answers
the amount of time it take to decrease from 1.00 m to 0.25 m
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Answers
Answer:
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Explanation:
In case of constant pressure, some of the energy is used to do work on the surrounding to keep pressure constant. Due to this, the total heat energy is less than in case of constant volume. In Case of constant Volume all of heat is available, produced in reaction because no work is done.
If we look at our data,we will find that process B has energy 23.3 KJ which is less than process A, the energy of which is 25.9 KJ. It means Process B is occurred at constant pressure condition and Process A has occurred at constant volume condition
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Answers
Answer:
B
Explanation:
Answer:
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Explanation: